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PR7000-0724

© Agilent Technologies, Inc. 2017

Published in the USA, September, 2017

5991-8363ENUC

Ordering Information

Cat. No.

Product Name

Reactions

MR-0031.024

HNPCC MASTR Plus

5

• MID (Molecular Identifiers) kits are necessary to complete the workflow

*3’ UTR of EPCAM only

Performance cont.

Graph presenting the read pair counts for all HNPCC MASTR Plus

amplicons, showing their outstanding uniform representation. To allow

comparison between samples, the read pair counts were normalized to

coverage of 100. The data are based on a 10 samples study, using the

Illumina MiSeq System.

0%

2%

4%

6%

8%

10%

12%

14%

16%

20%

18%

Read pair counts

MASTR Plus Workflow

Plan run

Multiplex PCR Quality Control

Universal PCR Quality Control

Multiplex PCR

Dilution

Universal PCR

Equimolar pooling

Mixing plexes of

single sample

Purification

Purification

Mixing Clean up

Pooling

Clean up

SEQUENCING

DATA ANALYSIS

NGS