S7804 datasheet(3/32 Pages) ETC

3 / 32 page

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TABLE OF CONTENTS

I. INTRODUCTION ..............................................................................1

Using this Manual ..................................................................................... 1

Background ............................................................................................... 1

Principles of the Technique....................................................................... 2

Fig 1: DNA Treatment with Sodium Bisulfite....................................... 3

II. KIT COMPONENTS .........................................................................4

Materials Required But Not Supplied ....................................................... 5

III. PROTOCOLS....................................................................................7

CpG WIZ™ p16 (S7800), p15 (S7802) and

E-cadherin (S7804) Amplification Kits ................................................. 7

Experimental Design............................................................................ 7

Fig. 2: Specificity of the CpG WIZ™ p16

Amplification Kit .................................................................................. 9

Amplification Protocol....................................................................... 10

CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit........... 12

Experimental Design.......................................................................... 12

Fig. 3: Specificity of the CpG WIZ™ Prader-Willi/

Angelman Amplification Kit ............................................................... 14

Amplification Protocol....................................................................... 15

IV.

DATA ANALYSIS ........................................................................... 18

CpG WIZ™ p16 (S7800), p15 (S7802) and

E-cadherin (S7804) Amplification Kits ............................................. 18

CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit ........... 19

V. TROUBLESHOOTING ....................................................................20

CpG WIZ™ p16 (S7800), p15 (S7802) and

E-cadherin (S7804) Amplification Kits .................................................. 20

CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit ........... 21

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