S7804 datasheet(16/32 Pages) ETC

16 / 32 page

f. Remove the tubes from the thermocycler block. From this point on, it is

important to designate separate pipettes and work areas for amplified vs.

unamplified samples. This prevents carry-over contamination of future DNA

samples with the amplified product.

STEP 3. Gel Electrophoresis

a. After the completion of PCR, add an appropriate amount of loading dye to

the sample and analyze 5 µL of the reaction on a 2% agarose, or a 10%

native acrylamide or other high resolution agarose gel. Use DNA markers

(100-300 bp range) to determine the size of PCR products.

b. After electrophoresis, stain the gel with ethidium bromide. Dilute the 10

mg/mL stock solution 1:10,000 in deionized water. Stain for 10-30 minutes

and destain for 10-30 minutes in deionized water at room temperature.

Note: Ethidium bromide is a known carcinogen. Exercise appropriate

caution and good lab practice when using this reagent.

CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit

Experimental

Design

Primer Sets

The CpG WIZ™ Prader-Willi/Angelman Amplification Kit contains primers

that can be used for analysis of DNA samples by MSP. However, the first step

of MSP is the bisulfite modification of the DNA samples. CpGenome™ DNA

Modification Kit (S7820), contains the reagents necessary to perform the

modification. This chemical modification creates the sequence differences

between the methylated and unmethylated DNA. The primer sets in the kit are

engineered to anneal to the DNA, based upon the sequence differences.

P (Paternal) Primer Set will anneal to unmethylated DNA that has undergone a

chemical modification.

M (Maternal) Primer Set will anneal to methylated DNA that has undergone a

chemical modification.

S7804 Datasheet(PDF) 16 Page - List of Unclassifed Manufacturers