S7804 datasheet(9/32 Pages) ETC

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Table 2: CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit

Components (color-coded microcentrifuge tubes)

Description

Amount

Storage

Conditions

P (paternal) primer set

5 µM each primer (25X)

70 µL (white cap)

-15°C to -25°C

M (maternal) primer set

5 µM each primer (25X)

70 µL (red cap)

-15°C to -25°C

Normal control DNA

0.1 µg/µL

20 µL (green cap)

-15°C to -25°C

10X PCR buffer

175 µL (blue cap)

-15°C to -25°C

Materials Required But Not Supplied

Equipment and Supplies

a. Thermocycler

b. Gel electrophoresis apparatus (vertical or horizontal)

c. Power Supply

d. Screw-cap tubes for PCR amplification

e. Aerosol-resistant pipette tips

f. Microcentrifuge (to 12,000 X g)

g. 302 nm UV transilluminator, camera and film

Reagents

a 2.5 mM dNTP mix (2.5 mM of each nucleotide)

b. Taq polymerase

Note: For S7806 (CpG WIZTM Prader-Willi/Angelman Amplification Kit),

the use of a "hot start" enzyme is strongly recommended. See Sec. III.

Protocols.

c. "Hot start" PCR reagents for S7800, S7802, and S7804 (see Sec. III.

Protocols).

S7804 Datasheet(PDF) 9 Page - List of Unclassifed Manufacturers