S7804 datasheet(18/32 Pages) ETC

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Figure 3: Specificity of the CpG WIZ™ Prader-Willi/Angleman Amplification

Kit.

Polyacrylamide gel analysis using the primers and the control DNA samples

included in the kit is shown. (Lane 1: P primer set with modified control DNA;

Lane 2: M primer set with modified control DNA; Lane 3: P primer set with

unmodified control DNA; Lane 4: M primer set with unmodified control DNA.

Marker Lane: 100 bp ladder)

Experiment Setup

The CpG WIZ™ Prader-Willi/Angelman Amplification Kit includes sufficient

reagents to analyze 25 samples with appropriate controls (70 PCR reactions).

For each batch of experimental DNA samples to be analyzed, the experimental

samples and the normal control DNA must first undergo bisulfite modification.

In the subsequent PCR reactions, the chemically modified DNA samples, both

experimental and control, are amplified using both the maternal (M) and

paternal (P) primer sets. In addition, a negative PCR control (i.e. no DNA) is

performed for each set of primers.

For example, a typical gel for the analysis of five experimental DNA samples

and proper controls includes a total of 14 lanes:

Lanes 1-2

Experimental sample 1 with P and M primers

Lanes 3-4

Experimental sample 2 with P and M primers

Lanes 5-6

Experimental sample 3 with P and M primers

S7804 Datasheet(PDF) 18 Page - List of Unclassifed Manufacturers